Cell Line Development Research Associate

Description

Cell Line Development Research Associate is involved in the creation of stable cell lines for the recombinant adeno-associated viral vector (rAAV) production processes . He/she executes experiments, produces relevant documentation, and works under supervision. He/she will be responsible for: · Culturing mammalian cells for viral production in shake flasks, spinner flasks, spin tubes, deep well and automation platforms. · Performing experiments in the development, optimization and scale-up of AAV producer cell lines. · Preparing process development protocols, data analysis summaries, technical reports, and other relevant process documentation. · Optimization of transfection and transduction protocols for newly engineered cell lines. · Maintaining an accurate laboratory notebook. · Ensuring work conforms to applicable quality and safety standards. · Participating on project teams (as needed). Education and Experience Requirements B.S. with 3-6 years of experience, MS.c with 2-5 years of relevant scientific or technical experience in the life sciences industry or equivalent · Experience in cell culture including materials, instruments and technologies is essential. · Experience with viral vector production is preferred . Key Skills, Abilities, and Competencies: Hands-on experience in mammalian cell culture production processes is a critical requirement. This includes small scale (shaker flasks, spinner flasks, spin tubes, deep well) and/or benchtop bioreactor scale. Experience with creation, analysis and characterization of new engineered cell lines and stability studies (subcloning, clonal expansion, transient transfection and expression screening) is a strong plus. Experience in molecular biology techniques (plasmid design, molecular cloning, SDS-PAGE/western blotting, qPCR, etc) is a strong plus. Automation and ELN experience is a strong plus. Effective communication skills (verbal, written, interpersonal). Industry experience is a plus but not required Basic Knowledge on AAV biology is a plus. Must be able to interact with cross-functional teams. Analytical and problem-solving skills. Ability to manage time effectively. Experience in laboratory automation hardware and software is a plus. Teamwork is a must. Must be able to lift 25 pounds. · Due to the inherent nature of cell culture, some weekend work and/or work on holidays may be needed. On occasion, long work hours may be required depending upon the experiment schedule. · Handling large volumes of cell culture or related materials, such as tens of liters, may be required. Also, several viral materials, including AAV may be involved in the work.