Jobs

Description

• Must have 2 years of Crispr experience.
• Molecular biology, qPCR, western blot. Proficiency in cell culture.  Primary cell lines. 
• Design guide RNA for a target, order a guide RNA, do the cell culture, grow cells, they do the electroporation, coordinate CRO work, submit samples on behalf of other Tas. 
• Them teach them single-cell-seq
• Everyday will be cell culture work; QPCR, western blot

Duties:

• Design, develop, and execute functional genomics assays, including CRISPR-based editing (e.g., RNP delivery, Perturb-seq), single-cell sequencing, spatial transcriptomics, and CITE-seq.
• Provide technical guidance and training to research scientists on FGSL technologies and techniques, including assay execution, troubleshooting, and resolution of technical issues;
• Documents and maintains detailed laboratory records in accordance with policies;
• Operates and maintains lab equipment in collaboration with other lab members;

Skills: 

• CRISPR assays - Must have personally designed guides, performed transfections/infections, selected edited cells, and validated knockouts/knockins
• Next-generation sequencing (NGS) - Hands-on library preparation experience (not just data analysis)
• Single cell technologies - Personal experience with sample prep, library generation, and quality control
• Standard molecular biology techniques - qPCR, Western blot, cloning, gel electrophoresis
• Cell culture - Proficient in maintaining multiple cell lines, transfections, and sterile technique
• Experience in automation is a plus;

Education:     

• Master’s degree from an accredited institution with 4+ years of experience in molecular, cell biology, or related scientific discipline.