-
| Posted On: Apr 26, 2024
Cambridge, MA 02139
18 Months, Contract
Job Details
-
Job Summary
Job Title: Biomolecule Production Scientist
Posted Date: Apr 26, 2024
Duration: 18 Months, Contract
Shift(s):
08:00 AM - 05:00 PM Pay Rate: 33.00 /Hourly
Description
BioMedicine Design (BMD) High-Throughput Protein Production (HTP) Group is seeking a highly motivated scientist to support the molecular biology & protein production workflows:
- DNA construct design/assembly, PCR, sample preparation for sequencing, DNA production & quantitation, mammalian cell culture & transfections, protein purification & quantitation, and automated liquid handling.
- The candidate will play a major role in a core group that consistently delivers hundreds of protein samples per week to research teams across the department.
- The candidate will be responsible for generating DNA constructs and performing high-throughput processes for macromolecule production (DNA, RNA, and protein).
- The candidate will also be responsible for updating the laboratory database and relaying data in organized presentations to project teams.
ROLE RESPONSIBILITIES
- DNA plasmid design & cloning
- Automated high throughput DNA purification
- High throughput molecular biology operations, including PCR, ligation reactions (Gibson & ITA), & DNA purification using liquid handling automation
- Sample preparation for sequencing
- DNA & RNA quantitation & QC
- Supporting transient mammalian transfections with DNA
- Organization and storage of DNA fragments/plasmids, including tracking using Genedata Biologics Software, as well as record keeping using electronic laboratory notebooks
- Close interaction with project teams
- Regular review of literature to maintain knowledge of scientific trends and industry processes, advance molecular biology and protein production methods
Hard Skills:
- PCR or molecular biology
- Open to Learn
Minimum education experience required:
- Bachelor’s degree (0-2 years’ experience) or Associate’s degree (1-3 years’ experience) with experience in Molecular Biology, Biochemistry, Biology, Biological Engineering or a related discipline along with a basic understanding of molecular biology and protein expression.
- Not looking for Master or above